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human pulmonary artery adventitia fibroblast medium  (AcceGen Biotechnology)


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    AcceGen Biotechnology human pulmonary artery adventitia fibroblast medium
    <t>Fibroblast</t> activation in 3D-bioprinted models of pulmonary arterial <t>adventitia.</t> A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.
    Human Pulmonary Artery Adventitia Fibroblast Medium, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+artery+adventitia+fibroblast+medium/Human+Pulmonary+Artery+Adventitia+Fibroblasts/bio_rxiv__2022__11__11__516188-121-6-12
    Average 93 stars, based on 1 article reviews
    human pulmonary artery adventitia fibroblast medium - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension"

    Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension

    Journal: bioRxiv

    doi: 10.1101/2022.11.11.516188

    Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.
    Figure Legend Snippet: Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.

    Techniques Used: Activation Assay, Expressing, Construct, MANN-WHITNEY, Immunostaining, Immunofluorescence

    Related Articles

    Construct:

    Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension
    Article Snippet: .. 3D-bioprinted constructs were cultured in complete human pulmonary artery adventitia fibroblast medium (AcceGen) supplemented with 2% FBS, 50 U mL −1 penicillin, 50 × 10 −6 M mL −1 streptomycin, and 0.5 μg mL −1 amphotericin B (Sigma) for metabolic activity experiments. ..

    Cell Culture:

    Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension
    Article Snippet: .. 3D-bioprinted constructs were cultured in complete human pulmonary artery adventitia fibroblast medium (AcceGen) supplemented with 2% FBS, 50 U mL −1 penicillin, 50 × 10 −6 M mL −1 streptomycin, and 0.5 μg mL −1 amphotericin B (Sigma) for metabolic activity experiments. ..

    Activity Assay:

    Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension
    Article Snippet: .. 3D-bioprinted constructs were cultured in complete human pulmonary artery adventitia fibroblast medium (AcceGen) supplemented with 2% FBS, 50 U mL −1 penicillin, 50 × 10 −6 M mL −1 streptomycin, and 0.5 μg mL −1 amphotericin B (Sigma) for metabolic activity experiments. ..



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    human pulmonary artery adventitia fibroblast medium  (AcceGen Biotechnology)
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    AcceGen Biotechnology human pulmonary artery adventitia fibroblast medium
    <t>Fibroblast</t> activation in 3D-bioprinted models of pulmonary arterial <t>adventitia.</t> A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.
    Human Pulmonary Artery Adventitia Fibroblast Medium, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+artery+adventitia+fibroblast+medium/Human+Pulmonary+Artery+Adventitia+Fibroblasts/bio_rxiv__2022__11__11__516188-121-6-12
    Average 93 stars, based on 1 article reviews
    human pulmonary artery adventitia fibroblast medium - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.

    Journal: bioRxiv

    Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension

    doi: 10.1101/2022.11.11.516188

    Figure Lengend Snippet: Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.

    Article Snippet: 3D-bioprinted constructs were cultured in complete human pulmonary artery adventitia fibroblast medium (AcceGen) supplemented with 2% FBS, 50 U mL −1 penicillin, 50 × 10 −6 M mL −1 streptomycin, and 0.5 μg mL −1 amphotericin B (Sigma) for metabolic activity experiments.

    Techniques: Activation Assay, Expressing, Construct, MANN-WHITNEY, Immunostaining, Immunofluorescence